Most peptide degradation happens on the bench, not in transit. This checklist covers solvent choice, volume maths, freeze-thaw and record-keeping.
Lyophilized peptides are stable for extended periods when stored correctly. Nearly all avoidable losses occur in the first ten minutes after the vial is opened.
Before you open the vial
- Allow the vial to reach room temperature — opening a cold vial draws in moisture.
- Confirm the lot number against the COA.
- Calculate solvent volume from net peptide content, not gross vial mass.
Reconstitution
- Store lyophilized vials at -4°F to 46°F (-20°C to 8°C), protected from light.
- Reconstitute with bacteriostatic water; add solvent slowly down the vial wall, never directly onto the pellet.
- Do not shake — swirl gently until fully dissolved.
- Once reconstituted, keep refrigerated and record the reconstitution date on the vial.
- Log the batch/lot number against the matching COA before any assay work begins.
Storage after reconstitution
| Condition | Practice |
|---|---|
| Temperature | Refrigerate at 2-8°C for short-term working solutions |
| Light | Amber vial or foil wrap; light exposure is cumulative |
| Freeze-thaw | Aliquot before freezing — repeated cycles are the main degradation source |
| Labelling | Date, concentration, solvent and lot number on every aliquot |
Every Palm Valley Peptides lot ships from the USA with same-day dispatch on cleared orders, HPLC-MS purity documentation, and a batch-matched certificate of analysis.
Bacteriostatic or sterile water?
Bacteriostatic water contains benzyl alcohol and supports multi-draw workflows; sterile water suits single-use preparations.
How much solvent should I add?
Enough to reach your target working concentration based on net peptide content — see the COA guide for the maths.
Research use only. Palm Valley Peptides supplies materials strictly for in vitro and laboratory research. Nothing in this article constitutes medical, therapeutic, or dosage advice for human or animal use.
